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Accumulating studies have demonstrated that non-coding RNAs (ncRNAs), functioning as important regulators of transcription and translation, are involved in the establishment and maintenance of pregnancy, especially the maternal immune adaptation process. The endometrial stromal cells (ESCs), trophoblast cells, and decidua immune cells that reside at the maternal-fetal interface are thought to play significant roles in normal pregnancy and pregnancy-associated diseases. Here, we reviewed the up-to-date evidence on how microRNA, long non-coding RNA, and circular RNA regulate ESCs, trophoblast cells, and immune cells and discussed the potential applications of these ncRNAs as diagnostic and therapeutic markers in pregnancy complications.
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Gravidez , Feminino , Humanos , MicroRNAs/genética , RNA Longo não Codificante/genética , RNA Circular/genética , Trofoblastos , Complicações na Gravidez/genéticaRESUMO
Objective:To explore the effects of fast-track surgery (FTS) combined with acupuncture at Neiguan acupoint on stress indicators and recovery quality in patients during anesthesia of craniocerebral surgery.Methods:According to block randomization method, 120 patients with craniocerebral trauma meeting inclusion criteria in the hospital were divided into three groups between January 2018 and December 2020, 40 in each group. The control group was given routine awakening intervention, intervention 1 group was given routine awakening intervention and acupuncture at Neiguan acupoint, and intervention 2 group was given FTS and treatment of intervention 1 group. At 10 min after entering the room, immediately after tracheal intubation and immediately after extubation, level of serum cortisol (COR) was detected by electrochemical luminescence apparatus. The level of serum dopamine was detected by ELISA. The blood pressure and heart rates were recorded. The recovery quality during anesthesia was compared, including recovery time, recovery time of spontaneous breathing, extubation time and recovery time of orientation. The cognitive function of patients was evaluated by Neurobehavioral Cognitive Status Examination (NSCE). The incidence of intestinal paralysis, nausea and vomiting, and hospitalization time were observed and recorded during treatment.Results:Immediately after tracheal intubation and extubation, systolic blood pressure in intervention 2 group, intervention 1 group and control group were significantly decreased ( F=12.03, 13.96), levels of serum COR ( F=91.40, 53.76), and dopamine ( F=29.72, 69.39) were significantly decreased ( P<0.01). The recovery time of orientation during anesthesia [(39.09±3.12)min vs. (41.57±3.54)min, (43.84±3.28)min, F=34.65] in intervention 2 group was significantly shorter than that of the intervention 1 group and control group ( P<0.01), and hospitalization time [(9.36±1.78)d vs. (10.92±1.81)d, (12.10±1.95)d, F=1.44] was significantly shorter than that of the intervention 1 group and control group ( P<0.05). Conclusion:The FTS combined with acupuncture at Neiguan acupoint can reduce stress level during anesthesia, improve recovery quality and relieve postoperative cognitive dysfunction in patients undergoing craniocerebral surgery.
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Objective@#To explore the bacteriological characteristics and bacterial resistance of children with purulent meningitis in the Shenmu Hospital, and to provide evidence for the rational use of antibiotics.@*Methods@#We retrospectively analyzed the clinical data and bacterial culture results of 43 children with purulent meningitis in our hospital from June 2009 to March 2017.@*Results@#There were 21 types of bacteria culture and drug susceptibility test: Gram-positive bacteria in 16 cases(76.19%), Gram negative bacilli in 5 cases(23.81%), including 8 staph infections, gambogic/lira micrococcus in 4 cases, excrement enterococcus in 1 case, streptococcus pneumoniae in 2 cases, produce mononuclear listeria in 1 case.All the Gram-negative bacilli were Escherichia coli.In cerebrospinal fluid culture, Gram-positive bacteria were significantly higher than Gram-negative bacteria, and the difference was statistically significant(χ2=7.813, P=0.005). There was no statistically significant difference in the number of Gram-positive cases between cerebrospinal fluid and blood culture pathogens(χ2=0.075, P=0.78). Gram positive coccus was generally resistant to penicillin, erythromycin and clindamycin, generation 1 cephalosporin antibiotic, including pneumococcal resistance rate was 100.0%, coagulase negative staphylococcus resistance rate was 87.5%, E.coli was resistant to ampicillin and levofloxacin.@*Conclusion@#In the pediatric treatment of our hospital, coagulase negative staphylococcus aureus and vine/Lila micrococcus is the important pathogenic bacteria that causes the children purulent meningitis, Escherichia coli is the only pathogenic bacteria of the Gram-negative bacterial meningitis.Vancomycin and linezolide are sensitive to cocci, and Escherichia coli is sensitive to antibiotics in the second and third generation of cephalosporins.
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Objective To investigate the levels of serum 25-hydroxy vitamin D in autumn and winter among children of Shenmu city,Shaanxi province,and to provide theoretical basis for prevention and treatment of rickets in this area.Methods From June 2016 to December 2016,a total of 277 children in the children's health clinic were sleceted.The morning fasting blood was extracted and serum levels of 25-hydroxy vitamin D were measured by enzyme-linked immunosorbent assay (ELISA).Results Among the 277 cases,there were 181 males and 96 females,including 163 infants(58.8％),75 toddlers(27.1％),24 preschool children(8.7％) and 15 school-going children(5.4％).The median (interquartile range) of serum 25-hydroxyl vitamin D was 33.21 (14.93)nmol/L.Among all children,4.3％ had severe vitamin D deficiency,15.9％ had moderate deficiency,57.0％ had mild deficiency,7.9％ had insufficient vitamin D levels,11.9％ had suitable vitamin D levels,while 2.9％ had excess of vitamin D.There was no statistically significant difference in the level of 25-hydroxyl vitamin D between different age groups(H =10.133,P ＞0.05),and different gender groups(Z =0.712,1.094,0.476,0.000,all P ＞0.05).The levels of serum 25-hydroxyl vitamin D in serum extracted in autumn were higher than those extracted in winter(75.96％ vs.90.75％,x2 =11.27,P ＜0.05).Conclusion There is significant deficiency of serum 25-hydroxyl vitamin D in Shenmu children,which significantly increased in winter.The outdoor activities should be strengthened and the vitamin D should be timely supplemented to prevent the occurrence of rickets.
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To explore the bacteriological characteristics and bacterial resistance of children with purulent meningitis in the Shenmu Hospital ,and to provide evidence for the rational use of antibiotics.Methods We retrospectively analyzed the clinical data and bacterial culture results of 43 children with purulent meningitis in our hospital from June 2009 to March 2017.Results There were 21 types of bacteria culture and drug susceptibility test :Gram-positive bacteria in 16 cases (76.19%), Gram negative bacilli in 5 cases (23.81%), including 8 staph infections,gambogic／lira micrococcus in 4 cases, excrement enterococcus in 1 case, streptococcus pneumoniae in 2 cases,produce mononuclear listeria in 1 case.All the Gram-negative bacilli were Escherichia coli.In cerebrospinal fluid culture,Gram-positive bacteria were significantly higher than Gram -negative bacteria,and the difference was statistically significant(χ2 ＝7.813,P ＝0.005).There was no statistically significant difference in the number of Gram-positive cases between cerebrospinal fluid and blood culture pathogens (χ2 ＝0.075,P＝0.78).Gram positive coccus was generally resistant to penicillin , erythromycin and clindamycin , generation 1 cephalosporin antibiotic , including pneumococcal resistance rate was 100.0%,coagulase negative staphylococcus resistance rate was 87.5%, E.coli was resistant to ampicillin and levofloxacin.Conclusion In the pediatric treatment of our hospital ,coagulase negative staphylococcus aureus and vine／Lila micrococcus is the important pathogenic bacteria that causes the children purulent meningitis, Escherichia coli is the only pathogenic bacteria of the Gram -negative bacterial meningitis. Vancomycin and linezolide are sensitive to cocci ,and Escherichia coli is sensitive to antibiotics in the second and third generation of cephalosporins.
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Objective To investigate the clinical manifestation,neuroimaging characteristics and follow-up results of chorea associated with non-ketotic hyperglycemia (C-H-BG).Methods Clinical data of six patients with C-H-BG were analyzed retrospectively.Results The average age of the six patients (five female and one male) was 65.8±13.9 years,All patients had acute-subacute course.Patients presented with choreic movements involving facial muscles (n=6) and unilateral (n=5) or bilateral limbs (n=1).The average value of plasma glucose at admission was 9.21 ± 5.15 mmol/L.All patients had significantly elevated HbA1c (14.1 ±3.5％).The brain MRI T1 imaging from all patients showed hyperintensity in the basal ganglia,putamen,lenticular nucleus and caudate nucleus.SWI imaging revealed corresponding hypointensity signal in the basal ganglia in three patients.Two patients had severe stenosis of the MCA.Five patients were followed up for an average of 16.2 ±7.2 months.Three patients died.Conclusion The high T1 hypertense lesions in the basal ganglia are the mainly radiological feature of C-H-BG which may be accompanied by SWI hytointense signals in the basal ganglia.
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Objective To investigate the clinical manifestation,neuroimaging characteristics and follow-up results of chorea associated with non-ketotic hyperglycemia (C-H-BG).Methods Clinical data of six patients with C-H-BG were analyzed retrospectively.Results The average age of the six patients (five female and one male) was 65.8±13.9 years,All patients had acute-subacute course.Patients presented with choreic movements involving facial muscles (n=6) and unilateral (n=5) or bilateral limbs (n=1).The average value of plasma glucose at admission was 9.21 ± 5.15 mmol/L.All patients had significantly elevated HbA1c (14.1 ±3.5％).The brain MRI T1 imaging from all patients showed hyperintensity in the basal ganglia,putamen,lenticular nucleus and caudate nucleus.SWI imaging revealed corresponding hypointensity signal in the basal ganglia in three patients.Two patients had severe stenosis of the MCA.Five patients were followed up for an average of 16.2 ±7.2 months.Three patients died.Conclusion The high T1 hypertense lesions in the basal ganglia are the mainly radiological feature of C-H-BG which may be accompanied by SWI hytointense signals in the basal ganglia.
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Objective To investigate the effect of salmeterol/fluticasone (SM/FP) on serum inflammatory factors in the treatment of bronchial asthma in children.Methods 80 children with bronchial asthma from January 2015 to October 2015 in department of pediatrics of first affiliated hospital of Zhejiang Chinese medicine university were selected and randomly divided into two groups.The control group were given routine clinical treatment, the experimental group were treated on the basis of the control group with salmeterol/fluticasone (SM/FP), for 4 weeks.The serum IL-2, IL-4, IFN-α, T-lymphocyte subsets and clinical efficacy between the two groups were compared.Results Compared with control group, the serum levels of IL-2 and IFN-γin experimental group were higher, IL-4 in experimental group was lower ( P <0.05 ); the serum CD3 +T, CD4 +T and CD4 +T /CD8 +T levels in experimental group were higher, the serum CD8 +T was lower than those in control group (P<0.05); the total efficiency of the experimental group (92.5%) was significantly higher than that of control group (75.0%) (P<0.05).Conclusion The salmeterol/fluticasone (SM/FP) has the good efficacy in the treatment of bronchial asthma in children, which could effectively regulate T lymphocyte subsets proportion and the level of cytokines.
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Objective To investigate the clinical, neuroimaging and serum risk factors of cerebral microbleeds (CMBs) in patients with ischemic stroke and find the associations between these risk factors and the location and num?bers of CMBs were also analyzed. Methods One hundred and fifty-three patients with acute ischemic stroke were re?cruited in this study and their data werewas retrospectively analyzed. All of the patients underwent MRI- susceptibility weighted imaging (SWI). The location and numbers of CMBs were recordedexamined. The severity of WMLs was assessed using the Fazekas scale. Logistic regressions were performed to find the predictors of the presence of CMBs. The relation?ships between these risk factors and the location and numbers of CMBs were also analyzed. Results Fifty-nine(38.6%) cases had at least one CMB. The frequency of cortical-subcortical, deep and infratentorial CMBs were 34.0%, 24.8%and 27.5%, respectively. Multivariate logistic regression showed that male sex, hypertension and moderate-to-severe deep white matter hyperintensities (DWMH) were independent risk factors of the presence of CMBs. Adjusted with age and sex, partial correlation showed that hypertension only correlated with the numbers of deep CMBs significantly (r=0.174, P=0.032). Moderate-to-severe DWMH significantly correlated with the numbers of cortical-subcortical and deep CMBs (r=0.285, P<0.001 and r=0.258, P=0.001, respectively). Conclusion Male sex, hypertension and moderate-to-severe DWMH were are independent risk factors of CMBs in patients with ischemic stroke. Hypertension correlates with Deep deep CMBs, while Moderatemoderate-to-severe DWMH correlates with cortical-subcortical and deep CMBs.
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Objective To investigate the clinical features and treatment of mycoplasma pneumonia in children,to facilitate clinical diagnosis and improve treatment.Methods A retrospective study was performed,and the data of 48 treated cases of mycoplasma pneumonia were analyzed.Results There were 32 males and 16 females, with male to female ratio of 2:1.Less than 1 year age reported 7 cases,accounted for 14%;1 -3 years,19 cases, accounted for 40%;>3-7 years 18 cases,accounted for 38%;and>7 to 10 years,4 cases,accounted for 8%.The youngest patient was 2 months and 10 days old,while the maximum age of patient reported was 9 years.Conclusion Highest prevalence of mycoplasma pneumonia was in 1 to 7 years age group children.Clinical manifestations were fever,cough and wheezing.Use of azithromycin,erythromycin or combination therapy all achieved good outcome.
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Purpose] To detect respiratory virus antigen by direct immunofluorescence method and provide evidence for early diagnosis of children with viral infection of the respiratory tract disease.[Methods] Select 398 cases of respiratory tract infection in hospitalized children, pharynx in exfoliated cells in respiratory syncytial virus by direct immunofluorescence(RSV), adenovirus(ADV), influenza virus type A(IFVA),influenza virus type B(IFVB), parainfluenza virus type 1(PIV1), parainfluenza virus type 2(PIV2) and parainflue-Nza virus type 3(PIV3) of 7 common viral antigens were detected, and carried on statistics analysis.[Results] In 398 cases of children with respiratory tract infection, 91 cases were positive, the positive rate was 22.86%. The highest for respiratory syncytial virus in 37 cases(40.66%) followed by parainfluenza virus type 3 in 19 cases(20.88%), influenza virus type B in 14 cases(15.38%); The positive rates of acute tonsillitis, capillary bronchitis, pneumonia, acute bronchitis and upper respiratory were 46.67%(14/30),40.00%(20/50), 26.67%(40/150), 18.42%(7/38), 7.69%(10/130) respectively; With the growth of age, the respiratory tract virus infection rate gradually decreased(P<0.05), one year old the viral infection rate was 76.92%;it occurred in the winter season of respiratory tract infection in children .[Conclusion] The main virus in children with acute respiratory infection in the local area is RSV. Virus infection decreases with the increase of age, treatment should be careful in the use of antibiotics.